Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Long-Term Intake of Uncaria rhynchophylla Reduces S100B and RAGE Protein Levels in Kainic Acid-Induced Epileptic Seizures Rats

doi: 10.1155/2017/9732854

Figure Lengend Snippet: Immunohistochemistry staining of S100B and hematoxylin and eosin (HE) staining in hippocampal sections from the PBS, KA, UR, and VA-pretreated groups. HE (blue) and S100B (brown) immunostaining in the whole hippocampus (a) and CA1 (b) in the PBS group. HE (blue) and S100B (brown) immunostaining in the whole hippocampus (c) and CA1 (d) areas in the KA group. HE (blue) and S100B (brown) immunostaining in the whole hippocampus (e) and CA1 (f) areas in the UR group. HE (blue) and S100B (brown) immunostaining in the whole hippocampus (g) and CA1 (h) areas in the VA group. The left panel was imaged at 40x magnification, and the right panel was imaged at 400x magnification.

Article Snippet: The sections were incubated overnight at 4°C with the primary antibody CCR2 (1 : 1000, Abcam, UK), mGluR3 (1 : 1000, Abcam, UK), MCP-1 (1 : 1000, Abcam, UK), RAGE (1 : 1000, Abcam, UK), S100B (1 : 1000, Novus Biologicals, USA), Actin (1 : 1000, Millipore, USA), 0.1% horse serum, and 0.1% Triton X-100 in PBS.

Techniques: Immunohistochemistry, Staining, Immunostaining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Long-Term Intake of Uncaria rhynchophylla Reduces S100B and RAGE Protein Levels in Kainic Acid-Induced Epileptic Seizures Rats

doi: 10.1155/2017/9732854

Figure Lengend Snippet: Western blot analysis of S100B, RAGE, mGluR3, MCP-1, and CCR-2 in the hippocampus from the PBS, KA, UR, and VA-pretreated groups. (a) S100B; (b) RAGE; (c) mGluR3; (d) MCP-1; (e) CCR-2. β -Actin was set as an internal control.

Article Snippet: The sections were incubated overnight at 4°C with the primary antibody CCR2 (1 : 1000, Abcam, UK), mGluR3 (1 : 1000, Abcam, UK), MCP-1 (1 : 1000, Abcam, UK), RAGE (1 : 1000, Abcam, UK), S100B (1 : 1000, Novus Biologicals, USA), Actin (1 : 1000, Millipore, USA), 0.1% horse serum, and 0.1% Triton X-100 in PBS.

Techniques: Western Blot

Journal: NPJ Regenerative Medicine

Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits

doi: 10.1038/s41536-021-00170-y

Figure Lengend Snippet: a GMSCs were encapsulated in 40 µl of 3D-collagen hydrogel at different concentrations (2, 4, 6 mg/mL) and a cell density of 2 × 10 6 /mL and then filled into AxoGuard Nerve protector or connector (NGC) (10 mm in length and 2 mm in inner diameter). Then, the constructs (NGC containing 3D collagen hydrogel encapsulated with GMSCs) were cultured for 24 h in complete α-MEM. Portions of this figure were made using templates from SMART SERVIER MEDICAL ART ( https://smart.servier.com ). b Before harvesting, the NGC constructs were labeled with 10 µM calcein-AM at 37 °C for 30 min. Cryosections were cut and the migrated cells labeled with calcein-AM (green color) in the wall matrix were observed under a fluorescence microscope. Nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI; blue). c Quantification of IF intensity of calcein-AM. d Cryosections of NGCs containing cell-free collagen hydrogel (Empty) or GMSC-laden collagen hydrogel at a concentration of 4 mg/mL (Cell-laden) were prepared for dual-color immunostaining for human nuclei (hNuclei; red color), SOX10 (red color), S-100β (green color) or GFAP (green color). Nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI; white or blue). Scale bar = 50 µm ( b , d ). *** p < 0.0001. Data represent the mean ± SD, n = 3 biological replicates. One-way ANOVA with the Tukey’s post test ( c ). NGC, nerve guidance conduit.

Article Snippet: Cryosections prepared from 3D-collagen gel or GMSC-seeded nerve conduits were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: p75 (mouse IgG, 1:200, Sigma), SOX-9 (rabbit IgG, 1:200, Cell Signal Tech), SOX-10 (mouse IgG, 1:200, R & D), S-100β (rabbit IgG, 1:200, Boster Biological Tech), NOTCH3 (rabbit IgG, 1:200, Abcam), HES1 (rabbit IgG, 1:200, Cell Signaling Tech), vinculin (mouse IgG, 1:400, Millipore), TRITC-conjugated phalloidin (1:400, Millipore), BDNF (rabbit IgG, 1:200, Abcam), GDNF (rabbit IgG, 1:200, Abcam), or NGF (rabbit IgG, 1:200, Abcam).

Techniques: Construct, Cell Culture, Labeling, Fluorescence, Microscopy, Concentration Assay, Immunostaining